Total Lipids with Short and Long Acyl Chains from Acholeplasma Form Nonlamellar Phases
نویسندگان
چکیده
منابع مشابه
Diffraction techniques for nonlamellar phases of phospholipids.
A neutron diffraction method applicable to nonlamellar phases of substrate-supported lipid membranes is described and validated. When prepared on a flat substrate, the resulting nonlamellar phases have layered symmetry which provides some advantages over powder diffraction for detailed structure determination. This approach recently led to the detection of a rhombohedral phase and a distorted h...
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The enveloped mycoplasmavirus MV-L2 and its host Acholeplasma laidlawii JA1 were used to study the ways in which changes in the membrane lipid bilayer affect virus adsorption. The physical state of the membranes was altered by (i) using viruses and bacteria with different membrane lipid acyl-chain compositions, (ii) using incorporation of cholesterol, and (iii) changing the temperature. Adsorpt...
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The partition coefficient Kp was measured for a headgroup-labeled phospholipid (12:0,12:0)-N-(7-nitrobenz-2oxa-1,3-diazol-4-yl)-PE (12-NBD-PE), equilibrated between LUV of a series of phosphatidylcholines (PC). Fluorescence resonance energy transfer between the 12-NBD-PE and a headgroup-rhodamine-labeled PE was used to find the equilibrium concentration of the 12-NBD-PE in the different LUV. Re...
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A Saccharomyces cerevisiae strain deficient in phosphatidylethanolamine methyltransferase that exhibits choline auxotrophy grew in the presence of dioctanoyl phosphatidylcholine (diC8PC). We isolated and characterized mutants defective in growth in the diC8PC-containing medium. Mutations in LEM3/ROS3 impaired growth in that medium, indicating that Lem3p is involved in the utilization of extrace...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1998
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(98)77730-6